{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-EB4O7OIT/febda3f9-da76-414a-875c-ecd67c2bc448/HTML","dcterms:extent":"25 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-EB4O7OIT/2e69281e-2372-4bf3-8997-47bd4b47446b/PDF","dcterms:extent":"660 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-EB4O7OIT/7892bef2-46af-4616-8fda-b927bcc822d8/TEXT","dcterms:extent":"22 KB"}],"edm:TimeSpan":{"@rdf:about":"1998-2025","edm:begin":{"@xml:lang":"en","#text":"1998"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-EB4O7OIT","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-KC6O72BG"},{"@xml:lang":"sl","#text":"Acta chimica slovenica"}],"dcterms:issued":"2006","dc:creator":["Jamnik, Andrej","Reščič, Jurij","Tomšič, Matija","Zalar, Petra"],"dc:format":[{"@xml:lang":"sl","#text":"številka:3"},{"@xml:lang":"sl","#text":"letnik:53"},{"@xml:lang":"sl","#text":"6 strani"},{"@xml:lang":"sl","#text":"str. 344-349"}],"dc:identifier":["ISSN:1318-0207","COBISSID:27968773","URN:URN:NBN:SI:doc-EB4O7OIT"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"dc:subject":[{"@xml:lang":"sl","#text":"albumini"},{"@xml:lang":"sl","#text":"človeški serum"},{"@xml:lang":"sl","#text":"fizikalna kemija"},{"@xml:lang":"sl","#text":"osmoza"},{"@xml:lang":"sl","#text":"osmozni tlak"},{"@xml:lang":"sl","#text":"proteini"},{"@xml:lang":"sl","#text":"raztopine"}],"dcterms:temporal":{"@rdf:resource":"1998-2025"},"dc:title":{"@xml:lang":"sl","#text":"Osmometry and small-angle x-ray scattering of human serum albumin in buffer solutions|"},"dc:description":[{"@xml:lang":"sl","#text":"Osmotic pressure measurements of human serum albumin (HSA) dissolved in 0.15M NaCl and in 0.1 M phosphate buffer at three values of pH are reported as a function of protein concentration. Experimentally obtained osmotic coefficients were well below one, indicating large deviations from ideality even for dilute protein solutions. This effect could be ascribed to formation of dimers or even higher aggregates in protein solution. The membrane equilibria measurements of osmotic pressures were therefore complemented by the small-angle X-ray scattering (SAXS) results that show no evidence of protein aggregates in solutions, leaving the question why is the osmotic coefficient so small unanswered"},{"@xml:lang":"sl","#text":"V predloženem delu so zbrani rezultati merjenj osmoznega tlaka raztopin proteina človeškega serumskega ablmina v 0,15 M NaCl in v 0,1 M fosfatnem pufru pri treh različnih vrednostih pH, in sicer kot funkcija koncentracije proteina. Eksperimentalno določeni osmozni koeficienti so znatno nižji od 1, kar nakazuje na velika odstopanja od idealnosti tudi pri razredčenih raztopinah proteina. Eden izmed možnih vzrokov za tako velika odstopanja je nastanek dimerov ali celo večjih agregatov. To domnevo smo preverili z uporabo metode ozkokotnega sipanja rentgenske svetlobe na vzorcih raztopin proteina, vendar obstoja dimerov nismo dokazali. Vprašanje, zakaj je osmozni koeficient raztopin človeškega serumskega albumina nizek, tako ostaja nepojasnjeno"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-EB4O7OIT","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-EB4O7OIT"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-EB4O7OIT/2e69281e-2372-4bf3-8997-47bd4b47446b/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-EB4O7OIT/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-EB4O7OIT"}}}}