{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-DRWT8GA5/d0d3868b-b9d9-4323-b152-15694ac8607c/PDF","dcterms:extent":"1892 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-DRWT8GA5/10442f88-00d5-49b8-932a-783639833d20/TEXT","dcterms:extent":"0 KB"}],"edm:TimeSpan":{"@rdf:about":"2000-2024","edm:begin":{"@xml:lang":"en","#text":"2000"},"edm:end":{"@xml:lang":"en","#text":"2024"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-DRWT8GA5","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/urn:nbn:si:spr-fzwbu7gu"},{"@xml:lang":"sl","#text":"Image analysis and stereology"}],"dcterms:issued":"2020","dc:creator":["Fink, Katja","Jensen, Jorgen","Kreft, Marko","Lobe Prebil, Mateja","Vardjan, Nina","Zorec, Robert"],"dc:format":[{"@xml:lang":"sl","#text":"številka:1"},{"@xml:lang":"sl","#text":"letnik:39"},{"@xml:lang":"sl","#text":"str. 25-32"}],"dc:identifier":["ISSN:1580-3139","COBISSID_HOST:27393795","URN:URN:NBN:SI:doc-DRWT8GA5"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Društvo za stereologijo in kvantitativno analizo slike, Medicinska fakulteta"},"dc:subject":[{"@xml:lang":"sl","#text":"adrenalin"},{"@xml:lang":"en","#text":"adrenaline"},{"@xml:lang":"sl","#text":"glikogen sintaza kinaza 3"},{"@xml:lang":"en","#text":"glycogen synthase kinase 3"},{"@xml:lang":"en","#text":"skeletal muscle fibre"},{"@xml:lang":"sl","#text":"skeletna mišična vlakna"}],"dcterms:temporal":{"@rdf:resource":"2000-2024"},"dc:title":{"@xml:lang":"sl","#text":"Increase in subcellular GSK-3 clusters in insulin- and adrenaline-treated differenti ated rat skeletal muscle fibres|"},"dc:description":{"@xml:lang":"sl","#text":"Glycogen synthase kinase 3 (GSK-3) plays an important role in m etabolic regulation in skeletal muscles, and both insulin and adrenaline stimulate GSK-3 phosphorylation . The aim of the present study was to study the effect of insulin and adrenaline on GSK-3 localisatio n in skeletal muscles. We characterized subcellular localization of GSK-3 signal protein in fully diffe rentiated muscle fibre by immunofluores- cence and confocal microscopy. We stimulated muscle fibres with insulin and/or adrenaline. Images were analysed by segmentation of si ngle central optical section of t he muscle. We found GSK-3 to be localised in clusters. The number of GSK-3 clusters and their average siz e were increased after stimulation with insulin and/or adrenaline. Average GSK-3 particle size is linea rly related to their quantity. We conclude that subcellular GSK-3 in isolate d skeletal muscle fibres is lo calized in clusters and clustering increased after stimulation with insulin and/or adrenaline"},"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-DRWT8GA5","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-DRWT8GA5"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-DRWT8GA5/d0d3868b-b9d9-4323-b152-15694ac8607c/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by-nc/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Društvo za stereologijo in kvantitativno analizo slike"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-DRWT8GA5/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-DRWT8GA5"}}}}