1892 KB0 KB200020242020Fink, KatjaJensen, JorgenKreft, MarkoLobe Prebil, MatejaVardjan, NinaZorec, Robertštevilka:1letnik:39str. 25-32ISSN:1580-3139COBISSID_HOST:27393795URN:URN:NBN:SI:doc-DRWT8GA5enDruštvo za stereologijo in kvantitativno analizo slike, Medicinska fakultetaImage analysis and stereologyadrenalinadrenalineglikogen sintaza kinaza 3glycogen synthase kinase 3skeletal muscle fibreskeletna mišična vlaknaIncrease in subcellular GSK-3 clusters in insulin- and adrenaline-treated differenti ated rat skeletal muscle fibres|Glycogen synthase kinase 3 (GSK-3) plays an important role in m etabolic regulation in skeletal muscles, and both insulin and adrenaline stimulate GSK-3 phosphorylation . The aim of the present study was to study the effect of insulin and adrenaline on GSK-3 localisatio n in skeletal muscles. We characterized subcellular localization of GSK-3 signal protein in fully diffe rentiated muscle fibre by immunofluores- cence and confocal microscopy. We stimulated muscle fibres with insulin and/or adrenaline. Images were analysed by segmentation of si ngle central optical section of t he muscle. We found GSK-3 to be localised in clusters. The number of GSK-3 clusters and their average siz e were increased after stimulation with insulin and/or adrenaline. Average GSK-3 particle size is linea rly related to their quantity. We conclude that subcellular GSK-3 in isolate d skeletal muscle fibres is lo calized in clusters and clustering increased after stimulation with insulin and/or adrenalineTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo za stereologijo in kvantitativno analizo slike