1024 KB33 KB200020242024Alibegović, ArminCvetko, ErikaLaubry, Lucija LuciePušnik, LukaSerša, IgorSnoj, ŽigaUmek, NejcUmek, Nejcletnik:43številka:ahead of printstr. 203-210DOI:10.5566/ias.3332ISSN:1580-3139COBISSID_HOST:214139907URN:URN:NBN:SI:doc-88N6DEX6enDruštvo za stereologijo in kvantitativno analizo slike, Medicinska fakultetaImage analysis and stereologyanaliza slikediffusion tensor imagingdifuzijsko tenzorsko slikanjefractional anisotropyfrakcijska anizotropijaimage analysisperiferni živecperineural tissueperinevralno tkivoperipheral nerveAnalysis-associated factors interfering with diffusion tensor indices of peripheral nerves|Diffusion tensor imaging (DTI) enables a non-invasive assessment of tissue architecture based on water diffusion. Advancements in technology have expanded the application of DTI from the central nervous system to the peripheral nervous system. High-resolution magnetic resonance imaging (MRI) systems have further enhanced the visualization of nerve compartments. This study investigates the influence of region of interest (ROI) selection on DTI indices in ex vivoperipheral nerves. Cadaveric median nerves (n=10) were harvested and immersed in fluorinated-carbon liquid, and 9-mm segments were independently scanned with a 9.4-T MRI system (scanning time 40 h). Diffusion-weighted signals were summed, and diffusion tensors were calculated for anatomical compartments using different delineation techniques. Following the initial scan, five samples were scanned for the second time using an identical protocol. The analysis showed that the inclusion of an inert background into the ROI significantly reduces fractional anisotropy (FA). Significant differences in diffusion tensors were observed between the initial and intermediate nerve portions, with FA and mean diffusivity (MD) differing by as much as 25.2% and 25.6%, respectively. Tracing the fascicles without the interfascicular epineurium exhibited an 8.8% lower FA compared to the delineations of the epineurium. Second MRI scans showed significant changes in diffusion tensors with higher eigenvalues D1-D3 and MD. In conclusion, the intermediate portions of the nerve demonstrated greater consistency in DTI indices and are therefore recommended for analysis over the initial portions. Using an inert liquid can minimize background effects, but the inherent diffusion properties of the tissue must be carefully considered. Prolonged scanning times can alter diffusion tensors, likely due to autolytic processes, underscoring the importance of consistent pre-scanning conditions and acquisition protocols. Additionally, maintaining consistency in delineations is paramount, regardless of whether the tracing method includes or excludes the interfascicular epineuriumTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo za stereologijo in kvantitativno analizo slike