{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-80ENNJJ5/e890ddf7-f1a5-40fb-85b7-e09e8e71145b/HTML","dcterms:extent":"19 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-80ENNJJ5/74e602b8-0cdb-4485-acbe-3a79c9a709a6/PDF","dcterms:extent":"356 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-80ENNJJ5/a0c8790e-cfbf-424b-b3c9-bbcdc68f428f/TEXT","dcterms:extent":"15 KB"}],"edm:TimeSpan":{"@rdf:about":"2000-2024","edm:begin":{"@xml:lang":"en","#text":"2000"},"edm:end":{"@xml:lang":"en","#text":"2024"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-80ENNJJ5","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/urn:nbn:si:spr-fzwbu7gu"},{"@xml:lang":"sl","#text":"Image analysis and stereology"}],"dcterms:issued":"2004","dc:creator":["Dahse, Regine","Kosmehl, Hartwig"],"dc:format":[{"@xml:lang":"sl","#text":"letnik:23"},{"@xml:lang":"sl","#text":"številka:3"},{"@xml:lang":"sl","#text":"5 strani"},{"@xml:lang":"sl","#text":"str. 153-157"}],"dc:identifier":["ISSN:1580-3139","COBISSID:20711897","URN:URN:NBN:SI:doc-80ENNJJ5"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Društvo za stereologijo in kvantitativno analizo slike, Medicinska fakulteta"},"dc:subject":[{"@xml:lang":"en","#text":"Coculture"},{"@xml:lang":"sl","#text":"Fibroblasti"},{"@xml:lang":"en","#text":"Fibroblasts"},{"@xml:lang":"en","#text":"Gene Expression"},{"@xml:lang":"sl","#text":"Gensko izražanje"},{"@xml:lang":"en","#text":"Immunohistochemistry"},{"@xml:lang":"sl","#text":"Imunohistokemija"},{"@xml:lang":"sl","#text":"izražanje genov"},{"@xml:lang":"sl","#text":"Kokultura"},{"@xml:lang":"sl","#text":"laserska mikrodisekcija"},{"@xml:lang":"en","#text":"Microscopy, Confocal"},{"@xml:lang":"sl","#text":"Mikroskopija konfokalna"},{"@xml:lang":"en","#text":"Neoplasm Invasiveness"},{"@xml:lang":"sl","#text":"Novotvorba, invazivnost"},{"@xml:lang":"en","#text":"Tumor Cells, Cultured"},{"@xml:lang":"sl","#text":"tumorji"},{"@xml:lang":"sl","#text":"Tumorske celične kulture"},{"@rdf:resource":"http://www.wikidata.org/entity/Q902045"}],"dcterms:temporal":{"@rdf:resource":"2000-2024"},"dc:title":{"@xml:lang":"sl","#text":"Cell separation and gene expression analysis in a tumor-stroma interaction model|"},"dc:description":{"@xml:lang":"sl","#text":"A novel technique for co-culturing and separating fibroblasts and carcinoma cells in a 2-D model of tumor stroma interaction is presented. The methodology is based on cell co-cultivation on an 1.35 microm thin membrane followed by rapid immunostaining and microdissection of the different cell compartments using a laser microdissection system (P.A.L.M. Microlaser Technologies AG, Germany). For identifying the tumor cell compartment, immunolabeling for a marker that is expressed only in epithelial tumor cells is performed. The RNA quality from the microdissected co-cultured cells was successfully proved by RT-PCR for a housekeeping gene transcript and for the laminin gamma 2 chain gene transcript used before in the tumor cell immunostaining. Laminin cDNA wasamplificable only in tumor cells and not in the co-cultivated fibroblasts indicating no cell-cross-contamination during microdissection. Microdissected tumor and stroma cells from the presented membrane based co-culture model can be used for gene expression profiling and DNA based analysis in the investigation of tumor-stroma interactions"},"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-80ENNJJ5","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-80ENNJJ5"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-80ENNJJ5/74e602b8-0cdb-4485-acbe-3a79c9a709a6/PDF"},"edm:rights":{"@rdf:resource":"http://rightsstatements.org/vocab/InC/1.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Društvo za stereologijo in kvantitativno analizo slike"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-80ENNJJ5/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-80ENNJJ5"}}}}