101 KB24 KB199820252013Barreteau, HéleneBlanot, DidierGobec, StanislavHrast, MartinaMengin-Lecreulx, DominiqueSosič, IzidorTurk, Samoštevilka:2letnik:60str. 294-299ISSN:1318-0207COBISSID:3477105URN:URN:NBN:SI:doc-5ZRWTVTEenSlovenian Chemical SocietySlovensko kemijsko društvoActa chimica slovenicaEscherichia coliMurFpeptidoglikaniprotibakterijske učinkovineStreptococcus pneumoniaevirtualno rešetanjezaviralna aktivnostBiochemical characterization of MurF from Streptococcus pneumoniae and the identification of a new MurF inhibitor through ligand-based virtual screening|MurF is an essential bacterial enzyme that is involved in the last intracellular stage of peptidoglycan biosynthesis, and therefore it has the potential to be exploited as a target for the development of new antibacterials. Here, we report on the expression, purification and biochemical characterization of MurF from an important pathogen, Streptococcus pneumoniae. Additionally, ligand-based virtual screening was successfully used and a new hit compound with micromolar inhibitory activities against MurF enzymes from S. pneumoniae and Escherichia coli was identifiedMurF je esencialen bakterijski encim, ki sodeluje pri zadnji znotrajcelični stopnji biosinteze peptidoglikana in je kot tak obetavna tarča za razvoj novihprotibakterijskih učinkovin. V tem prispevku poročamo o uspešni ekspresiji, izolaciji in biokemijski karakterizaciji encima MurF iz pomembnega patogenega seva Streptococcus pneumoniae. Poleg tega smo tudi uspešno uporabili virtualno rešetanje na osnovi liganda in odkrili novo spojino zadetek z zaviralno aktivnostjo v mikromolarnem območju na encima MurFiz S. pneumoniae in Escherichia coliTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko kemijsko društvo