19 KB330 KB13 KB200020242005Demjénová, ErnaJanáček, JiříKaren, PetrKubínová, LucieRadochová, BarbaraSchwarzerová, KateřinaTomori, Zoltánletnik:24številka:35 stranistr. 181-185ISSN:1580-3139COBISSID:20442073URN:URN:NBN:SI:doc-5DTGQTZEenDruštvo za stereologijo in kvantitativno analizo slike, Medicinska fakultetaImage analysis and stereologycitologijaEndoplasmic ReticulumEndoplazemski retikulumendoplazmatski retikulumImage Processing, Computer-AssistedMicroscopy, ConfocalmikroskopijaMikroskopija konfokalnaSlika, obdelava z računalnikomstereologijaTobaccoTobakUltrastructureAnalysis of endoplasmic reticulum of tobacco cells using confocal microscopy|Image analysis techniques for preprocessing, segmentation and estimation of geometrical characteristics of fiber-like structures from 2-D or 3-D images captured by a confocal microscope are presented. Methods are demonstrated on fiber-like biological structure: endoplasmic reticulum (ER) of tobacco cells. In the presented analysis of 2-D images of ER before and after the treatment of latrunculin B, ER and ER tubules were segmented and the area density of ER as well as the length density of ER tubules in the cell cortical layer were estimated by automatic image analysis algorithms. Images of 3-D arrangement ofER were reconstructed and rendered by various visualization techniquesTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo za stereologijo in kvantitativno analizo slike