24 KB328 KB16 KB200020242001Moorman, Antoon FMRuijter, Jan MSoufan, Alexandre Tvan den Hoff, Mauriceletnik:20številka:36 stranistr. 193-198COBISSID:14252761ISSN:1580-3139URN:URN:NBN:SI:doc-4PIXP17MenDruštvo za stereologijo in kvantitativno analizo slike, Medicinska fakultetaImage analysis and stereology3D grafikaanatomijaAnatomy and histologyembriologijaEmbryologyGrowth and developmentHeartImage processing, computer-assistedMicroscopy, confocalmikroskopijaMikroskopija konfokalnaMiokardMitotic indexMitotični indeksMyocardiumobdelava slikPodganeRatsSlika, obdelava s pomočjo računalnikaSrceQuantitative 3D reconstructions as identification tool in heart development|A method for displaying quantitative information in 3D reconstructions of the embryonic heart was developed to investigate spatial distributions of cell division and cell density. The method utilizes serial sections to extract morphological as well as quantitative data. The morphological data are used toreconstruct the embryonic heart and the quantitative data are classified andsuperimposed on the resulting reconstruction. The bias, which would result from size differences between cell populaions, was investigated. If present, it would influence the absolute number of particles (nuclei) per volume, although the classification applied on the reconstruction displaying the mitotic fraction remains unchanged. Although the reconstruction displaying thelocal densities is influenced by the bias, less than 2.5% of the regions ismissclassifiedTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo za stereologijo in kvantitativno analizo slike