207 KB71 KB199420251999Hermann, Dejanštevilka:3letnik:8str. 339-365ISSN:0025-8121COBISSID:10343641URN:URN:NBN:SI:doc-2D9UHKIEslMedicinski razglediMedicinski razglediAntigen-antibody complexComplicationsdiagnostikaFluorescent antibody technique, directglomerulonefritisGlomerulonephritisImmunologyimunologijainfekcijeledvicePathologyStreptococcal infectionsHistomorfologija in imunohistologija postinfekcijskega glomerulonefritisa v odvisnosti od trajanja bolezni in vrste okužbe| Histomorphology and immunohistology of postinfectious glomerulonephritis in relation to the duration of the disease and type of infection|In addition to the classical picture of endocapillary proliferative and exudative glomerulonephritis, patients with acute postinfectious glomerulonephritis may manifest a broad spectrum of various histomorphologicaland immunohistological changes which may reflect the stage of the disease, their responsivness to the disease and/or the specific type ofinfection. The data on the correlation between histomorphological changes and immune deposits are still sparse. The purpose of this study was to evaluate these, most probably key relationships in the pathogenesis related tothe duration of the disease and type of infection. This retrospective study involved 113 kidney biopsies from 91 men and 22 women with the diagnosis of postinfectious glomerulonephritis. The tissue samples were examined by standard light and immunofluorescent microscopy techniques. Mesangial, endocapillary and mesangiocapillary proliferation, exudation, tubulo-interstitial changes, vascular lesions and glomerular immune deposits were evaluated semiquantitatively using the generally recognized nephropatological methods. Extracapillary proliferation and global and segmentglomerulosclerosis were evaluated as a percentage of the affected glomeruli. Immune deposits demonstrated the following three immunofluorescent patterns: garland, starry sky and mesangialPri bolnikih z akutnim postinfekcijskim glomerulonefritisom ugotavljajo poleg klasične histomorfološke slike endokapilarnega proliferacijskega in eksudacijskega glomerulonefritisa širok spekter histomorfološkega in imunohistološkega izražanja bolezni, ki je lahko posledica stadija bolezni, individualne odzivnosti in/ali razlik v vrsti okužbe. V dosegljivi literaturi so odnosi med histomorfološkimi spremembami in odlagami imunskih reaktantov opisani pomanjkljivo. Cilj raziskave je zato bil natančneje opredeliti le-te, v patogenezi verjetno ključne odnose v odvisnosti od trajanja bolezni in vrsteokužbe. Retrospektivna raziskava je vključevala 113 ledvičnih biopsij 91 moških in 22 žensk, ki so izpolnjevali klinićne kriterije za diagnozo akutnegapostinfekcijskega glomerulonefritisa. Vzorci ledvičnega tkiva so bili obdelani po standardni svetlobno in imunofluorescentno mikroskopski tehnologiji. V skladu z uveljavljeno metodologijo v nefropatologiji smo semikvantitativno ocenili stopnjo mezangijske, endokapilarne in mezangiokapilarne proliferacije, eksudacijske reakcije, tubulo-intersticijskihsprememb in sprememb na žilah ter glomerulnih imunskih odlag. Ekstrakapilarno proliferacijo, globalno in segmentno glomerulosklerozo smo ocenili v odstotkih prizadetih glomerulov. Glede na obliko imunskih odlag smo ugotavljali tri imunofluoresoenhie vzorce: ogrličast, vzorec zvezdnatega neba in mezangijskiTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaDruštvo Medicinski razgledi