{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-28GNWRQ0/feb542c1-dfee-4bdd-8a2a-272785a70894/HTML","dcterms:extent":"15 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-28GNWRQ0/23e9e8bc-b416-4ac7-b41f-27150d3e0508/PDF","dcterms:extent":"121 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-28GNWRQ0/cb4003c5-dc2a-4e5f-a7b4-82370ab2a9f3/TEXT","dcterms:extent":"14 KB"}],"edm:TimeSpan":{"@rdf:about":"1998-2025","edm:begin":{"@xml:lang":"en","#text":"1998"},"edm:end":{"@xml:lang":"en","#text":"2025"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-28GNWRQ0","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-KC6O72BG"},{"@xml:lang":"sl","#text":"Acta chimica slovenica"}],"dcterms:issued":"2009","dc:creator":["Kos, Janko","Pečar Fonović, Urša"],"dc:format":[{"@xml:lang":"sl","#text":"številka:4"},{"@xml:lang":"sl","#text":"letnik:56"},{"@xml:lang":"sl","#text":"str. 985-988"}],"dc:identifier":["ISSN:1318-0207","COBISSID:2730353","URN:URN:NBN:SI:doc-28GNWRQ0"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"dc:subject":[{"@xml:lang":"sl","#text":"biokemija"},{"@xml:lang":"sl","#text":"cisteinske proteaze"},{"@xml:lang":"sl","#text":"čiščenje"},{"@xml:lang":"sl","#text":"izolacija"},{"@xml:lang":"sl","#text":"katepsin"}],"dcterms:temporal":{"@rdf:resource":"1998-2025"},"dc:title":{"@xml:lang":"sl","#text":"Efficient removal of cathepsin L from active cathepsin X using immunoprecipitation technique|"},"dc:description":[{"@xml:lang":"sl","#text":"Cathepsin X is a cysteine protease which is involved in various important physiological and pathological processes. For the purpose of biochemical and structural studies, pure and active cathepsin X is required without contamination with other related proteases. Recombinant cathepsin X was obtained by expression in methylotropic yeast Pichia pastoris. During purification, cathepsin X has to be activated with cysteine protease cathepsinL, however, separation methods, used so far, can not completely remove cathepsin L from the activated cathepsin X. Here we describe a new purification procedure which provides active recombinant cathepsin X without the presence of residual cathepsin L"},{"@xml:lang":"sl","#text":"Katepsin X je cisteinska proteaza, ki je udele`ena v razli~nih pomembnih fiziolo{kih in patolo{kih procesih. Za potrebe biokemijskih in strukturnih raziskav je potreben ~ist in aktiven katepsin X brez vsebnosti sorodnih proteaz. Rekombinantni katepsin X smo izrazili v metilotropni kvasovki Pichia pastoris. V postopku izolacije je potrebno katepsin X aktivirati s cisteinsko proteazo katepsinom L, vendar z do sedaj objavljenimi metodami ni bilo mogo~e popolnoma lo~iti katepsina L in aktivnega katepsina X. V ~lanku opisujemo novometodo izolacije, s katero dobimo aktiven katepsin X brez prisotnosti katepsina L"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-28GNWRQ0","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-28GNWRQ0"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-28GNWRQ0/23e9e8bc-b416-4ac7-b41f-27150d3e0508/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Slovensko kemijsko društvo"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-28GNWRQ0/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-28GNWRQ0"}}}}