34 KB366 KB32 KB199820252011Jazbec Križman, PetraKotnik, DarjaKrižman, MitjaProšek, MirkoŠmidovnik, Andrejštevilka:2letnik:58str. 203-211ISSN:1318-0207COBISSID:4786202URN:URN:NBN:SI:doc-1RO6OLGMenSlovenian Chemical SocietySlovensko kemijsko društvoActa chimica slovenicaanalizna kemijabiološki vzorcicarbohydrate and lipid analysishranahyphenated techniquesion chromatography-mass spectrometrykromatografijamasna spektroskopijathin-layer chromatography-gas chromatographythin-layer chromatography-mass spectrometryOverview of the development and application of the hyphenated techniques in nutritional analysis|The development of some sensitive assays for quantitative nutritional analysis with an emphasis on selected hyphenated analytical techniques is reviewed in the present paper. The majority of work is dedicated to reviewing the development of analytical tools for routine analysis of carbohydrates and lipids in biological samples, many of them introduced in our laboratory. Handling biological matrices, where endogenous compounds can mask the analyte of interest or where the occurrence of the coelution effect of several compounds present in different amounts hinders the analyte's peak integration, is a major challenge. To overcome this challenge, hyphenated techniques have become widespread in laboratory practice. Some of these techniques are reviewed, with special attention given to an effective on-line interface for thin-layer chromatography-mass spectrometry and on-line coupling thin-layer chromatography-gas chromatography. Recently introduced an on-line coupling of ion chromatograph and hybrid RF/DC quadrupole-linear ion trap mass spectrometer represent an analytical tool for the solution of bioanalytical problems. Developed methods using ion chromatography- pulsed amperometric detection and ion chromatography-mass spectrometry techniques for the quantitative evaluation of sugars are presented. This paper represents basic contributions of our research work connected with some of modern hyphenated techniques. However, this review is restricted to the published papers to be significant developments or improvements during the last three decadesV prispevku je podan pregled razvoja nekaterih analiznih metod za kvantitativno določanje spojin na prehrambenem področju, s poudarkom na izbranih sklopljenih analiznih tehnikah. Večji del je posvečen pregledu razvoja analiznih orodij za rutinske analize ogljikovih hidratov in maščob v bioloških vzorcih, mnoge med njimi vpeljane v našem laboratoriju. Rokovanje z biološkimi vzorci predstavlja velik izziv, saj lahko endogene spojine zakrijejo tarčni analit in kjer koelucijski učinki večih spojin, prisotnih v različnih količinah, lahko ovirajo integracijo vrhov izbranih analitov. Da premostimo omenjene izzive, so bile v laboratorijsko prakso uvedene sklopljene analizne tehnike. Predstavljene so nekatere rešitve, s posebnim poudarkom na učinkovitem vmesniku med tenkoplastno kromatografijo in masno spektroskopijo ter povezava med tenkoplastno kromatografijo in plinsko kromatografijo. Za premagovanje bioanalitskih problemov je bila pred kratkim na Kemijskem inštitutu v Ljubljani inštalirana sklopitev ionskega kromatografa in masnega spektrometra, hibrid RF/DC kvadrupola in linearne ionske pasti. Predstavljene so razvite metode za določevanje ogljikovih hidratov, uporabljajoč ionsko kromatografijo v povezavi z masno spektrometrijo in ionsko kromatografijo s pulzno amperometrično detekcijo. Obstoječ pregledni članek predstavlja naše raziskovalno delo na področju modernih sklopljenih tehnik. V članku so izpostavljeni signifikantni doprinosi na področju analitike ogljikovih hidratov in lipidov z izbranimi sklopljenimi tehnikamiTEXTznanstveno časopisjejournalsSlovenian National E-content AggregatorNational and University Library of SloveniaSlovensko kemijsko društvo