{"?xml":{"@version":"1.0"},"edm:RDF":{"@xmlns:dc":"http://purl.org/dc/elements/1.1/","@xmlns:edm":"http://www.europeana.eu/schemas/edm/","@xmlns:wgs84_pos":"http://www.w3.org/2003/01/geo/wgs84_pos","@xmlns:foaf":"http://xmlns.com/foaf/0.1/","@xmlns:rdaGr2":"http://rdvocab.info/ElementsGr2","@xmlns:oai":"http://www.openarchives.org/OAI/2.0/","@xmlns:owl":"http://www.w3.org/2002/07/owl#","@xmlns:rdf":"http://www.w3.org/1999/02/22-rdf-syntax-ns#","@xmlns:ore":"http://www.openarchives.org/ore/terms/","@xmlns:skos":"http://www.w3.org/2004/02/skos/core#","@xmlns:dcterms":"http://purl.org/dc/terms/","edm:WebResource":[{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-0DXKZ73P/a7a8c432-19ea-4d11-9eb3-237aecdff598/PDF","dcterms:extent":"160 KB"},{"@rdf:about":"http://www.dlib.si/stream/URN:NBN:SI:DOC-0DXKZ73P/c3420efd-f022-487e-ad95-a045e7091a24/TEXT","dcterms:extent":"0 KB"}],"edm:TimeSpan":{"@rdf:about":"2002-2026","edm:begin":{"@xml:lang":"en","#text":"2002"},"edm:end":{"@xml:lang":"en","#text":"2026"}},"edm:ProvidedCHO":{"@rdf:about":"URN:NBN:SI:DOC-0DXKZ73P","dcterms:isPartOf":[{"@rdf:resource":"https://www.dlib.si/details/URN:NBN:SI:spr-ETYMF1IG"},{"@xml:lang":"sl","#text":"Acta biologica slovenica"}],"dcterms:issued":"2025","dc:creator":["Bratkovič, Tomaž","Ivanovski, Andrej"],"dc:format":[{"@xml:lang":"sl","#text":"številka:4"},{"@xml:lang":"sl","#text":"letnik:68"},{"@xml:lang":"sl","#text":"str. 126-133"}],"dc:identifier":["DOI:10.14720/abs.68.4.22623","ISSN:1854-3073","COBISSID_HOST:264924931","URN:URN:NBN:SI:doc-0DXKZ73P"],"dc:language":"en","dc:publisher":{"@xml:lang":"sl","#text":"Društvo biologov Slovenije"},"dc:subject":[{"@xml:lang":"sl","#text":"Bacillus subtilis"},{"@xml:lang":"en","#text":"continuous bioprocessing"},{"@xml:lang":"en","#text":"downstream processing"},{"@xml:lang":"sl","#text":"kontinuirno bioprocesiranje"},{"@xml:lang":"sl","#text":"pripravljalni procesi"},{"@xml:lang":"sl","#text":"proizvodnja rekombinantnih proteinov"},{"@xml:lang":"en","#text":"recombinant protein production"},{"@xml:lang":"sl","#text":"Rekombinantni proteini"},{"@xml:lang":"en","#text":"upstream processing"},{"@xml:lang":"sl","#text":"zaključni postopki"}],"dcterms:temporal":{"@rdf:resource":"2002-2026"},"dc:title":{"@xml:lang":"sl","#text":"Continuous bioprocessing for recombinant protein production in Bacillus subtilis| Kontinuirno bioprocesništvo za proizvodnjo rekombinantnih proteinov v Bacillus subtilis| opportunities and challenges| priložnosti in izzivi|"},"dc:description":[{"@xml:lang":"sl","#text":"The Gram-positive bacterium Bacillus subtilis has long been used for industrial protein production owing to its well-characterised genetic background, capacity for high-density fermentation, and effective protein secretion. With the growing global demand for recombinant proteins across various sectors, there is an increasing need for more efficient and sustainable protein production methods. This review explores the application of continuous bioprocessing for recombinant protein production using B. subtilis, critically evaluating current technologies and discussing their potential for integration into streamlined, integrated biomanufacturing framework. We primarily focus on transgenic B. subtilis, however the solutions for continuous fermentation and protein isolation/purification are the same for both endogenous and heterologous (i.e., recombinant) proteins"},{"@xml:lang":"sl","#text":"Po Gramu pozitivno bakterijo Bacillus subtilis že dolgo uporabljamo v industrijski proizvodnji proteinov. K temu prispevajo njena dobra raziskana genetska zasnova, možnost doseganja visoke biomase med fermentacijo in učinkovito izločanje proteinov. Zaradi naraščajočega svetovnega povpraševanja po rekombinantnih proteinih v različnih sektorjih, se povečuje tudi potreba po učinkovitejših in trajnostnih metodah proizvodnje proteinov. V članku predstavljamo potencial kontinuirnega bioprocesništva pri proizvodnji rekombinantnih proteinov z uporabo B. subtilis. Ponujamo pregled obstoječih tehnologij in njihovega potenciala za vključitev v integrirani koncept učinkovitejše bioproizvodnje rekombinantnih proteinov. Čeprav se prispevek primarno posveča transgenim bakterijam B. subtilis, so rešitve kontinuirne fermentacije in izolacije/čiščenja proteinov enake za endogene in heterologne (tj. rekombinantne) proteine"}],"edm:type":"TEXT","dc:type":[{"@xml:lang":"sl","#text":"znanstveno časopisje"},{"@xml:lang":"en","#text":"journals"},{"@rdf:resource":"http://www.wikidata.org/entity/Q361785"}]},"ore:Aggregation":{"@rdf:about":"http://www.dlib.si/?URN=URN:NBN:SI:DOC-0DXKZ73P","edm:aggregatedCHO":{"@rdf:resource":"URN:NBN:SI:DOC-0DXKZ73P"},"edm:isShownBy":{"@rdf:resource":"http://www.dlib.si/stream/URN:NBN:SI:DOC-0DXKZ73P/a7a8c432-19ea-4d11-9eb3-237aecdff598/PDF"},"edm:rights":{"@rdf:resource":"http://creativecommons.org/licenses/by-sa/4.0/"},"edm:provider":"Slovenian National E-content Aggregator","edm:intermediateProvider":{"@xml:lang":"en","#text":"National and University Library of Slovenia"},"edm:dataProvider":{"@xml:lang":"sl","#text":"Društvo biologov Slovenije"},"edm:object":{"@rdf:resource":"http://www.dlib.si/streamdb/URN:NBN:SI:DOC-0DXKZ73P/maxi/edm"},"edm:isShownAt":{"@rdf:resource":"http://www.dlib.si/details/URN:NBN:SI:DOC-0DXKZ73P"}}}}