113 research article The impact of anaemia on treatment outcome in patients with squamous cell carcinoma of anal canal and anal margin Irena Oblak12, Monika Cesnjevar2, Mitja Anzic2, Jasna But Hadzic1, Ajra Secerov Ermenc1, Franc Anderluh1, Vaneja Velenik12, Ana Jeromen1, Peter Korosec1 1 Department of Radiotherapy, Institute of Oncology Ljubljana, Ljubljana, Slovenia 2 Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia Radiol Oncol 2016; 50(1): 113-120. Received 26 November 2014 Accepted 22 December 2014 Correspondence to: Assist. Prof. Irena Oblak, M.D., Ph.D., Department of Radiotherapy, Institute of Oncology Ljubljana, Ljubljana, Slovenia. Phone: +386 1 5879 515; Fax: +386 1 5878 304; E-mail: ioblak@onko-i.si Disclosure: No potential conflicts of interest were disclosed. Background. Radiochemotherapy is the main treatment for patients with squamous cell carcinoma of the anal canal. Anaemia is reported to have adverse effect on survival in cancer patients. The aim of the study was to evaluate the influence of anaemia on radiochemotherapy treatment outcome in patients with squamous cell carcinoma of the anal canal. Patients and methods. One hundred consecutive patients with histologically confirmed squamous cell carcinoma of the anal canal were treated radically with 3-dimensional conformal or intensity-modulated radiation therapy followed by brachytherapy or external beam radiotherapy boost and with concurrent mitomycin C and 5-fluorouracil. The influence on survival of pre-treatment, mean on-treatment and end-of-treatment haemoglobin (Hb) concentrations was studied. Results. The 5-year locoregional control, disease free survival, disease specific survival and overall survival rates for all patients were 72%, 71%, 77% and 62%, respectively. In univariate analysis, patients with pre-treatment and end-of-treatment Hb > 120 g/L survived statistically significantly better compared to patients with Hb < 120 g/L. Patients with mean on-treatment Hb > 120 g/L only had statistically significant better locoregional control and overall survival than patients with Hb < 120 g/L. In multivariate analysis, independent prognostic factors were pre-treatment Hb (> 120 g/L vs. < 120 g/L) for overall survival (hazard ratio [HR] = 0.419, 95% confidence interval [CI] = 0.190-0.927, p = 0.032) and stage (I & II vs. III) for disease specific (HR = 3.523, 95% CI = 1.375-9.026, p = 0.009) and overall survival (HR = 2.230, 95% CI = 1.167-4.264, p = 0.015). Conclusions. The pre-treatment, mean on-treatment and end-of-treatment Hb concentration > 120 g/L carried better prognosis for patients for with squamous cell carcinoma of the anal canal treated with radiochemotherapy. The pre-treatment Hb > 120 g/L was an independent prognostic factor for overall survival of patients with anal canal cancer. Key words: anaemia; anal canal squamous cell carcinoma; radiochemotherapy Introduction Squamous cell anal cancer is a rare tumour which represents 1.5% of gastrointestinal cancers, but in Slovenia only 0.5%.1-5 Despite its infrequent occur- rence its incidence is increasing.4 Women are more commonly affected than men.3-6 Causal factors in the anal canal cancer are usually associated with human papilloma virus (HPV) infection (being the most important risk factor), human immuno- Radiol Oncol 2016; 50(1): 113-120. doi:10.1515/raon-2015-0015 114 Oblak I et al. / Anaemia and anal canal carcinoma deficiency virus (HIV) infection, anal intercourse, higher lifetime number of sexual partners, genital warts and cigarette smoking.36-8 Anal canal cancer is predominantly a loco-regional disease, because it metastasizes in less than 10% of patients, mainly to lungs and liver.6 The management of anal canal cancer has undergone an interesting transformation over the course of the past three decades. With the report by Nigro et al. in 1974 it shifted from abdomino-perineal resection with or without inguinal lymph node dissection to radical radiochemotherapy.910 Radiochemotherapy with 5-fluorouracil and mitomycin C, nowadays being the main treatment, results in complete tumour response in 70-90% and has a 5-year survival rate of 60-70%, leaving surgery only as a salvage treatment for tumours that do not respond to radiochemotherapy or recur.47 Anal margin cancers are classified as skin tumours and small tumours can be treated by surgery, while tumours T2 or larger should be treated with definitive radiochemotherapy.11 Radiotherapy as well as chemotherapy is known to be more efficacious in the presence of oxygen than in hypoxic conditions.12-15 Tumours are more hypoxic than the surrounding normal tissue.13 Anaemia, present in 75% of cancer patients, could increase the proportion of hypoxic tumour cells.13 Hypoxia is widely recognized as a major factor leading to the resistance of tumour cells to radiotherapy, but several mechanisms may also cause cells in the hypoxic region to be resistant to anticancer drugs.16 The influence of anaemia on the outcome of treatment was first recognized in 1940s in cervical cancer patients and later in patients with other tumours such as head and neck squamous cell carcinoma, carcinoma of the lungs, bladder, prostate and anus.71718 The purpose of present study was to evaluate the influence of anaemia on radiochemotherapy treatment outcome in patients with squamous cell carcinoma of the anal canal. Patients and methods One hundred consecutive patients (60 females and 40 males) with histologically confirmed squamous cell carcinoma of the anal canal were included in the retrospective study. They were treated at the Institute of Oncology Ljubljana from January 2003 till June 2013. For performance status (PS) the scoring system of the World Health Organization (WHO) was used19, and for TNM staging the criteria of the Union for International Cancer Control (UICC).20 Pre-treatment evaluation Pre-treatment evaluation consisted of physical and digital rectal examination, rectoscopy with biopsy and fine needle aspiration biopsy of enlarged inguinal lymph nodes, also ultrasound-guided, like in other cancer patients.21 Imaging included chest X-ray or computer tomography (CT) of chest, abdominal ultrasound (US) or CT and magnetic resonance imaging (MRI) of the pelvis. Laboratory tests included serum chemistry and complete blood count in all patients, and testing for HIV infection in high-risk patients. A multidisciplinary team consisting of a surgeon, a radiation oncologist and a medical oncologist decided the treatment for each patient. Radiotherapy Clinical target volume (CTV) consisted of the tumour volume with a safety margin of 2-2.5 cm and the regional lymph node areas. An additional margin of 1 cm was applied to the CTV for the planning target volume (PTV). Initial tumour borders were marked with tattoo. Positron emission tomography with computed tomography (PET-CT) was used as an aid in treatment planning. The treatment schedule for external beam radiotherapy (EBRT) consisted of 3-dimensional (3-D) conformal photon beam radiotherapy or intensity modulated radiotherapy (IMRT) with individual field arrangement. The total dose was 45 Gy in 25 fractions, 5-times weekly with 15 MV photon beam linear accelerator, plus a boost 10-15 Gy with interstitial pulsed-dose rate brachytherapy if tumour size was less than 5 cm. Metal needles were homogeneously implanted through a per-ineal template according to the rules of the Paris system. In tumours larger than 5 cm or in N2-3 disease, the boost was delivered with EBRT. CTV (brachytherapy/EBRT) of the boost corresponded to the initial gross tumour extension. In cases with positive inguinal lymph nodes, inguinal areas were boosted with electrons to a total dose of 59.4 Gy. When IMRT technique was used, inguinal lymph nodes were involved in CTV and PTV and irradiated to the same total dose of 59.4 Gy. If the tumour involved or crossed the external anal sphincter, this area was covered with a 1 cm thick gelatinous bolus to raise the dose at the surface to at least 95% of the planned dose. Radiol Oncol 2016; 50(1): 113-120. 114 Oblak I et al. / Anaemia and anal canal carcinoma Chemotherapy Chemotherapy protocol consisted of 2 cycles of 96-hour continuous infusion of 5-fluorouracil with a daily dose of 1000 mg/m2 of body surface in the first and fifth week of radiotherapy. On day 1 the patients also received a bolus of mitomycin C in a dose of 10 mg/m2. Since 2006, we administered peroral cytostatic capecitabine in a dose of 825 mg/m2, twice daily, to cooperative patients with good performance status and without important comorbidities. First dose of capecitabine was administered one hour before the irradiation and the second dose 12 hours after. In cases of severe treatment toxicity according to common toxicity criteria22 radiotherapy and/or chemotherapy was modified according to the patient's general condition and laboratory findings or was even temporarily interrupted. Follow-up During treatment, the patients were examined weekly to assess acute toxicity and compliance with radiochemotherapy, and complete blood count and serum biochemistry were performed as well. The first post treatment examination was performed six weeks after the completion of radio-chemotherapy, and then every 2-3 months for the first 2 years and every 6 months in the following 3 years. When tumour response was incomplete, patients were examined every 6 weeks over a period of 4 months after the end of the treatment. In this period we performed all necessary investigations to prove tumour viability or its progression and in such cases surgery (abdomino-perineal resection) was recommended. Tumour response was evaluated according to the WHO criteria.19 TABLE 1. Patients' and tumours' characteristics Characteristics Gender female male Mean age [range] Performance status (WHO] 0 1 2 3 Tumour type Carcinoma of the anal canal Carcinoma of the anal margin Tumour histology Basaloid Squamous TNM N0 N1 N2 N3 T1 9 0 1 0 T2 36 6 1 0 T3 19 10 3 1 T4 1 1 7 5 Tumour stage IIIA No. of patients 60 40 63 (34-87] 76 20 3 1 72 28 12 88 IIIB 9 55 17 19 WHO = World Health Organization Statistical analysis The survival estimates were carried out by using the Kaplan-Meier method23 and a log rank test24 was used to test the differences in survival between subgroups. The end points of survival analysis were defined as follows: loco-regional control (LRC) as the time interval from the beginning of the treatment to the appearance of local and/or regional progression; disease-free survival (DFS) as the time interval from the beginning of the treatment to the appearance of local and/or regional progression and/or appearance of distant metastases; disease-specific survival (DSS) as the time interval from the beginning of the treatment to the death because of cancer; and overall survival (OS) as the time interval from the beginning of the treatment to the death due to any cause. For multivariate analysis, Cox proportional hazard model (with "Enter method") was used.25 All statistical tests were two-sided and a P-value of p < 0.05 was considered statistically significant. Statistical analyses were performed by using SPSS version 22 (Chicago, IL). Radiol Oncol 2016; 50(1): 113-120. 114 Oblak I et al. / Anaemia and anal canal carcinoma TABLE 2. Haemoglobin (Hb) values in subgroups of patients Hb (g/L) No. of patients Median Hb (g/L) Hb range (g/L) Pre-treatment Hb 128 86- 169 > 120 g/L 69 136 122- -169 < 120 g/L 31 107 86- 120 Mean on-treatment Hb 127 96- 157 > 120 g/L 67 134 121- -157 < 120 g/L 33 113 96- 119 End-of-treatment Hb 121 77- 159 > 120 g/L 46 134 121- -159 < 120 g/L 54 114 77- 120 Ethical consideration The study was carried out according to the Helsinki Declaration (1964, with later amendments) and according to the European Council Convention on Protection of Human Rights in Bio-Medicine (Oviedo, 1997). It was approved by the Institutional Review Board Committee and by the National Committee for Medical Ethics, Ministry of Health, of the Republic of Slovenia. Results The study was closed on February 15, 2014. Median follow-up time of all patients was 52 months (range: 1-129 months) and 72 months (range: 6-129 months ) for the survivors. On the day of analysis, 59 patients were alive, 22 patients died of anal canal cancer, 15 patients died of other causes and in 4 patients the cause of death was unknown. Characteristics of patients and tumours are shown in Table 1. Characteristics of Hb values in subgroup of patients are shown in Table 2. Ninety-two patients (92%) completed their treatment according to the protocol. In 8 patients the treatment was modified: three did not receive chemotherapy due to significant comorbidities (is-chemic heart disease or significant hepatopathy); in 1 patient chemotherapy was terminated due to acute side effects (chest pain due to a suspected is-chemic event) and in 1 patient due to febrile neu-tropenia. One patient refused further treatment after 45 Gy and 1 patient refused chemotherapy. One patient received concurrent chemotherapy with cisplatin due to simultaneous treatment of the synchronous oropharyngeal cancer. Median duration of radiochemotherapy was 1.9 months (range: 1-3.7 months). Fifty-six patients received brachytherapy boost with medial dose of 18.5 Gy (range: 10-25 Gy) or EBRT boost with medial dose of 14.4 Gy (range: 9-14.4 Gy). Capecitabine was used instead of 5-fluorouracil in 25 patients. Tumour response to treatment Complete clinical remission of the disease was achieved in 80 patients. The tumour disappeared within six weeks after the treatment completion in 73 patients, and within 4 months in 7 patients. One of them was operated on because of presumed persistent disease, yet the pathologist did not find disease residues. Of the remaining 20 patients, in 1 patient the disease progressed during treatment, 9 patients had APR performed and 2 patients had inguinal lymphadenectomy due to recurrence in inguinal lymph nodes; 8 patients had inoperable residual disease. Survival The 5-year LRC, DFS, DSS and OS rates for all patients were 72%, 71%, 77% and 62%, respectively. Univariate analysis for survival according to the Hb level and other parameters is shown in Table 3. In multivariate analysis, pre-treatment Hb (> 120 g/L vs. < 120 g/L) was an independent prognostic factor only for OS (hazard ratio [HR]= 0.419, 95% confidence interval [CI] = 0.190-0.927, p = 0.032) and stage (I & II vs. III) for DSS (HR = 3.523, 95% CI = 1.375-9.026, p = 0.009) and OS (HR = 2.230, 95% CI = 1.167-4.264, p = 0.015). Patients' age, gender, tumour site, type of radiotherapy boost (tele- or brachytherapy) and type of chemotherapy (5-fluorouracil or capecitabine) did not have an influence on survival. Haemoglobin concentration during treatment In the group of patients with Hb > 120 g/L the mean Hb concentration during the treatment slightly but not significantly decreased (mean pre-treatment Hb = 139 g/L, mean end-of-treatment Hb = 125 g/L). However in the group of patients with Hb < 120 g/L it slightly increased (mean pre-treatment Hb = 106 g/L, mean end-of-treatment Hb = 113 g/L). One third of patients had low iron levels and received iron preparations. Nine patients received blood transfusion due to a drop in their Hb concentration below 100 g/L. Radiol Oncol 2016; 50(1): 113-120. 114 Oblak I et al. / Anaemia and anal canal carcinoma Acute side effects None of the patients died because of acute side effects. Most grade 3 side effects were caused by radiodermatitis. Serious, life-threatening infections were observed in 3 patients: 2 patients experienced severe pneumonia that requested transfer to the intensive care unit and 1 patient developed febrile neutropenia which required termination of radiochemotherapy. One patient developed severe stomatitis and needed parenteral nutrition. In 1 patient, serious diarrhoea developed, which required hospitalization. Frequency and intensity of acute side effects are shown in Table 4. Discussion Survival rates of our patients and the profile and frequency of acute side effects are similar to the results of other researchers.2726-29 There was no difference in survival of anal canal and anal margin cancer patients. The survival rate of patients with higher pre-treatment and end-of treatment Hb concentrations was generally better, compared to those patients with lower Hb concentrations, yet only pre-treatment Hb concentration was an independent prognostic factor for OS. Patients with mean on-treatment Hb > 120 g/L only had statistically significant better LRC and OS than patients with Hb < 120 g/L. Many authors found that anaemic patients respond worse to radiotherapy and/or chemotherapy and have worse survival rates.2'8'121315-18'30-41 There is convincing evidence of a correlation between Hb concentration and tumour oxygenation in various kinds of tumours.42 Nordsmark's et al. comparison of pre-treatment Hb with pre-treatment tumour pO2 measurements in head and neck cancer showed a quadratic regression correlation between Hb concentration and median pO2.43 Tumours of anaemic patients are consequently more hypoxic and more resistant to radiotherapy (and chemotherapy).16 The National Comprehensive Cancer Network (NCCN) guidelines recommend the use of blood transfusion in symptomatic patients with Hb concentration <100 g/L to improve oxygen delivery to the tumour.44 Nine patients in our study received blood transfusion. They had statistically significant worse OS than other patients. The conclusions about beneficial effect of transfusion in our study cannot be made because the patients who received transfusion were few. The contribution to low survival of other un- TABLE 3. Univariate analysis of survival of patients at 5 years by Hb level, tumour-, patients-, and treatment characteristics Characteristics LRC DFS DSS Pre-treatment Hb > 120 g/L < 120 g/L Mean on-treatment Hb > 120 g/L < 120 g/L End-of-treatment Hb > 120 g/L < 120 g/L Performance status PS 0 PS 1-3 Tumour stage T1-3 T4 Lymph node involvement no yes Overall disease stage I / II IIIA / IIIB Histologic tumour type basaloid squamous Tumour site anal canal anal margin Blood transfusion no yes Overall radiation time < 1,08 months > 1,08 months Operation no yes 69 31 67 33 46 54 76 24 86 14 65 35 64 36 12 88 72 28 91 9 29 71 73 27 79% 57% 78% 60% 82% 63% 73% 69% 75% 50% 79% 59% 79% 59% 100% 69% 81% 72% 89% 64% 89% 29% 77% 57% 76% 60% 80% 63% 73% 64% 75% 44% 79% 56% 79% 57% 100% 67% 68% 81% 71% 89% 63% 88% 29% 85% 56% 82% 67% P = 0.037 P = 0.054 P = 0.081 89% 65% P = 0.022 P = 0.037 P = 0.011 80% 66% P = 0.480 P = 0.283 P = 0.231 84% 38% 87% 60% 87% 61% 100% 74% P = 0.030 P = 0.024 P = 0.051 78% 73% 78% 93% 69% 88% 52% P = 0.000 P < 0.000 P = 0.001 OS P = 0.011 P = 0.017 P = 0.003 P = 0.054 P = 0.015 P = 0.000 P = 0.032 P = 0.017 P = 0.000 P = 0.044 P = 0.025 P = 0.000 P = 0.250 P = 0.212 P = 0.994 P = 0.993 P = 0.950 P = 0.333 P = 0.015 P = 0.011 P = 0.012 73% 39% P = 0.000 50% P = 0.007 75% 49% P = 0.003 72% 34% P = 0.000 68% 25% P = 0.001 70% 48% P = 0.000 70% 49% P = 0.000 100% 57% P = 0.014 62% 61% P = 0.738 64% 0% P = 0.044 83% 51% P = 0.012 69% 45% P = 0.018 DFS = disease-free survival; DSS = disease-specific survival; Hb = haemoglobin; LRC = loco-regional control; N = number of patients; OS = overall survival n Radiol Oncol 2016; 50(1): 113-120. 114 Oblak I et al. / Anaemia and anal canal carcinoma TABLE 4. Acute treatment toxicities Toxicity Grade 0 1 2 3 4 Total Stomatitis 68 12 10 9 1 100 Nausea, vomiting 79 9 9 3 0 100 Diarrhoea 57 17 12 13 1 100 Hand-foot syndrome* 22 0 1 2 0 25 Radiodermatitis 10 12 13 64 1 100 Infection 51 14 23 9 3 100 Leucocyte count 37 31 20 10 2 100 Haemoglobin level 43 44 11 2 0 100 Platelet count 58 36 3 3 0 100 * Only in patients treated with capecitabine favourable factors, which are often combined with anaemia, was not possible to assess. The reports in the literature of the influence of transfusions on the outcome are not consistent. Some authors found favourable effect45, some found none4647 and some found unfavourable effect.232 It is possible that a better oxygen delivery is not sufficient to improve oxygenation of a tumour with high oxygen consumption.30-35 Moreover, anaemic patients are assumed to have a more aggressive disease from the start.35 46 Immune suppression in patients could also play a part (7, 35).7'35 The use of erythropoetin is controversial due to the possible effect on tumour growth143348, however, only in the subpopulation of patients whose tumours expressed erythropoetin receptors.49 Another potential mechanism by which erythro-poetin therapy may result in negative outcomes in cancer patients is through promotion of thrombo-vascular events.50 Therefore, it was not used in our patients. De Los Santos et al. believe the connection between anaemia and hypoxia is complex; therefore, it is not clear whether transfusion or erythro-poetin do patients any favour.51 The Hb concentration during treatment progressively decreased, which is in agreement with other reports.2,7,17 18,30-33 46 At the beginning of treatment, 31% of our patients were anaemic, and at the end 54%. That should cause more hypoxia in the tumour. It is possible that a decreased delivery of oxygen to the tumour due to of Hb drop dur- ing the treatment is partially counterbalanced by the reoxygenation due to shrinkage of the tumour and does not influence very much the outcome. In some patients with Hb < 120 g/L it was possible to raise the mean Hb level by the blood transfusion or by iron preparations. The significance of mean on-treatment Hb concentration and end-of-treatment Hb concentration is less clear. Some authors found a positive effect of higher mean on-treatment Hb concentration on treatment outcome2,15,18,32,33,35 and some found a positive effect of higher end-of-treatment Hb concentration on treatment outcome35,36, while others found no influence on outcome of either mean- or end- of-treatment Hb level.31 In our patients, the mean- or end- of treatment Hb levels had less influence on survival compared to the pre-treatment values of Hb concentration. Our study showed that pre-treatment Hb was an important independent prognostic factor for overall survival in patients with squamous cell carcinoma of the anal canal and anal margin treated with radiochemotherapy, which is in agreement with findings of most other authors. Mean on-treatment Hb and end-of-treatment Hb do not seem to have much influence on survival. Because of a small number of patients who needed blood transfusion its influence on survival could not be assessed in our study. References 1. 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