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Mikropropagacija ogrožene vrste Hladnikia pastinacifolia - 
preliminarni rezultati
Micropropagation of Endangered Hladnikia pastinacifolia - 
preliminary results
t e r e z i j a c i r i N g e r
1
, M i T j a k a l i g a r i č
1
, j a n a aM B r o ž i č -do l i n Š e k
1,2
1
  University of Maribor, Faculty of Natural Sciences and Mathematics, Koroška 160 
Maribor, Slovenia; rezka.ciringer@uni-mb.si
2
  University of Maribor, Faculty of Education, Koroška 160, Maribor, Slovenia
Hladnikia pastinacifolia Rchb. is endemic species in the area of Slovenia with limited 
distributional range of 4 km2 within the Trnovski gozd. H. pastinacifolia is a red list species 
in Slovenia, listed also in the Appendix II of Habitat Directive. Its habitats are considered as 
endangered, in the case of screes also priority habitats (Appendix I of Habitat Directives). 
An efficient protocol for rapid multiplication of H. pastinacifolia for ex situ conservation is 
described, due to possible degradation of its natural habitats.
We used solid Murashige and Skoog medium for establishment of tissue culture with 
and without growth regulators: 6-benzylaminopurine (0-20 μM BAP), 2-isopentinyl 
adenine (0-20 μM 2-iP), thidiazuron (0-10 μM TDZ), kinetin (0-20 μM K), zeatin 
(0-20 μM K), naphthalene acetic acid (0-1 μM NAA), indol-3-butyric acid (0-3 μM IBA). 
Development of axillary shoots was most successful with combination of growth regulators 
respectively: 10 μM BAP/1 μM IBA, follows 2 μM BAP/3 μM IBA, 10 μM BAP/3 μM IBA 
and 0.5  μM TDZ. The protocol can be applied also for research in the field of population 
genetics and ecology of species.
t e r e z i j a c i r i N g e r e t a l .: Mikropropagacija ogrožene vrste Hladnikia pastinacifolia…